A Colorimetric Method for Quantifying Cis and Trans Alkenes Using an Indicator Displacement Assay

A colorimetric indicator displacement assay (IDA) amenable to high-throughput experimentation was developed determine the percentage of cis and trans alkenes. Using 96-well plates two steps are performed: a reaction plate for dihydroxylation alkenes followed by an IDA screening consisting boronic acid. The generates either erythro or threo vicinal diols from alkenes, depending upon their syn- anti-addition mechanisms. Threo preferentially associate with acid due creation more stable boronate esters, thus displacing greater extent. generality protocol demonstrated using seven sets Blind mixtures were made, resulting in average error ±2 % implementing E2 Wittig reactions gave errors ±3 %. Furthermore, we variants which color may be tuned optimize response human eye.